Morphologically distinct colonies were transferred

5 of loline minimal media and incubated at 28 for 72 Bacterial strains showing abundant growth in loline minimal medium were selected for further analysis and identified by 16S rRNA sequencing.Molecular methodsBacterial strains were inoculated into 10 tryptic soy broth for 24 and total genomic DNA was extracted using a DNeasy Plant kit (Qiagen, Valencia, CA, USA). Reactions were performed in a thermal cycler (Peltier Thermocycler PTL 200, MJ Research, Watertown, MA, USA) with an initial denaturation step at 95 for 3 followed by 27 cycles at 95 for 30 53 for 30 and 72 for 1 with final extension at 72 for 10 Amplified PCR products were purified using a Qiagen PCR purification kit. Further characterization of strain 7R was conducted with specific 16S rRNA primers for the Burkholderia cepacia complex: PC SSF and PCSSR (specific for B.

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